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cell culture ectocervical  (ATCC)


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    ATCC cell culture ectocervical
    Cell Culture Ectocervical, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+ectocervical/Ect1%2FE6E7/ppr0860131-170-3-7
    Average 96 stars, based on 350 article reviews
    cell culture ectocervical - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: Gardnerella Vaginalis Alters Cervicovaginal Epithelial Cell Function Through Epithelial Cell-type Specific Immune Responses
    Article Snippet: .. Materials And Methods Cell Culture Ectocervical (Ect/E6E7, AATC# CRL-2614) (Ecto), endocervical (End1/E6E7, AATC# CRL-2615) (Endo) and vaginal (VK2/E6E7, ATCC# CRL-2616) (VK2) human epithelial cell lines (American Type Culture Collection, Manassas, VA) were cultured in Keratinocyte-Serum Free Media (K-SFM) supplemented with 0.1 ng/mL epidermal growth factor and 50 ug/mL bovine pituitary extract (Gibco, Life Technologies), 100 U/mL penicillin, and 100 μg/mL of streptomycin at 37oC in a 5% CO2 humidi ed incubator. .. TLR2 (NF-kB-SEAP / KI-IL-8 Lucia) dual-reporter human embryonic kidney (HEK) 293 cells (Invivogen, San Diego, CA), a TLR2 reporter cell line, expresses the human TLR2 gene, an NF-kB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene and the Lucia luciferase reporter gene under the Page 5/32 control of the endogenous IL-8 promoter.

    Article Title: Vaginal microbes alter epithelial transcriptome and induce epigenomic modifications providing insight into mechanisms for susceptibility to adverse reproductive outcomes
    Article Snippet: .. Materials and Methods Cell Culture Ectocervical (Ect/E6E7, ATCC# CRL-2614) (Ecto), endocervical (End1/E6E7, ATCC# CRL-2615) (Endo) and vaginal (VK2/E6E7, ATCC# CRL-2616) (VK2) human epithelial cell lines (American Type Culture Collection, Manassas, VA) were cultured in Keratinocyte-Serum Free Media (KSFM) supplemented with 0.1 ng/mL epidermal growth factor and 50 μg/mL bovine pituitary extract (Gibco, Life Technologies), 100 U/mL penicillin, and 100 μg/mL of streptomycin at 37oC in a 5% CO2 humidi ed incubator. .. The monocyte cell lines, THP1-Null2 (Invivogen) and THP1-KO-NLRP3 (Invivogen), which have a biallelic KO of the Nterminal region of the NLRP3 gene, were cultured according to the manufacture's protocol.

    Article Title: Vaginal microbes alter epithelial transcriptomic and epigenomic modifications providing insight into the molecular mechanisms for susceptibility to adverse reproductive outcomes
    Article Snippet: .. Materials and Methods Cell Culture Ectocervical (Ect/E6E7, AATC# CRL-2614) (Ecto), endocervical (End1/E6E7, AATC# CRL-2615) (Endo) and vaginal (VK2/E6E7, ATCC# CRL-2616) (VK2) human epithelial cell lines (American Type Culture Collection, Manassas, VA) were cultured in Keratinocyte-Serum Free Media (K-SFM) supplemented with 0.1 ng/mL epidermal growth factor and 50 ug/mL bovine pituitary extract (Gibco, Life Technologies), 100 U/mL penicillin, and 100 μg/mL of streptomycin at 37oC in a 5% CO2 humidi ed incubator. .. The monocyte cell lines, THP1-Null2 (Invivogen) and THP1-KO-NLRP3 (Invivogen), which have a biallelic KO of the Nterminal region of the NLRP3 gene, were cultured according to the manufacture's protocol.

    Article Title: Postintegration HIV-1 Infection of Cervical Epithelial Cells Mediates Contact-Dependent Productive Infection of T Cells
    Article Snippet: .. Cell Culture Ectocervical (Ect1/E6E7) and endocervical (End1/E6E7) cell lines were obtained from the American Type Culture Collection and grown in cell-specific media as indicated by the manufacturer. .. Primary CD4+ T cells were isolated from blood using CD4+ T-cell isolation kit II (Miltenyi Biotec Inc.) and activated with 4 μg/mL phytohemagglutinin and 50 U/mL interleukin-2 for 72 hours in Roswell Park Memorial Institute complete media with penicillin streptomycin and 10% fetal bovine serum.



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    In vitro tenofovir-dp formation in PBMCs and epithelial cells

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Single-dose pharmacokinetics of tenofovir alafenamide and its active metabolite in the mucosal tissues

    doi: 10.1093/jac/dkx064

    Figure Lengend Snippet: In vitro tenofovir-dp formation in PBMCs and epithelial cells

    Article Snippet: Cell culture methods Ectocervical (Ect1/E6E7) ATCC ® CRL-2614TM and vaginal (VK2/E6E7) ATCC ® CRL-2616TM epithelial cell lines were obtained and sub-cultured for >4 passes after the initial thaw.

    Techniques: In Vitro

    Human ectocervical cells (ECT1) were pretreated with fibronectin to block integrin-ligand interactions and then infected with MHV68; 24h after infection, MHV68 was found to be partially blocked from cells treated with fibronectin (Fibronectin) compared to cells treated with MHV68 but no fibronectin (NT)(3 independent experiments) (A). Western blot analysis showed integrin alpha 3 and beta 1 were upregulated in the pregnant cervix of infected mice (B) n=3. Antibodies blocking integrin alpha 3 (MHV68 + anti-integrin a3 ab) partially blocked MHV68 infection, while treatment with non specific IgG (MHV68 + IgG) did not block MHV68 infection as compared to cells only treated with MHV68 (MHV68 only) (Representative of 3 independent experiments with a minimum of 3 animals per group) (C). Significance is denoted by bars and is p <.05.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Viral infection of the pregnant cervix predisposes to ascending bacterial infection

    doi: 10.4049/jimmunol.1300661

    Figure Lengend Snippet: Human ectocervical cells (ECT1) were pretreated with fibronectin to block integrin-ligand interactions and then infected with MHV68; 24h after infection, MHV68 was found to be partially blocked from cells treated with fibronectin (Fibronectin) compared to cells treated with MHV68 but no fibronectin (NT)(3 independent experiments) (A). Western blot analysis showed integrin alpha 3 and beta 1 were upregulated in the pregnant cervix of infected mice (B) n=3. Antibodies blocking integrin alpha 3 (MHV68 + anti-integrin a3 ab) partially blocked MHV68 infection, while treatment with non specific IgG (MHV68 + IgG) did not block MHV68 infection as compared to cells only treated with MHV68 (MHV68 only) (Representative of 3 independent experiments with a minimum of 3 animals per group) (C). Significance is denoted by bars and is p <.05.

    Article Snippet: Cell culture Immortalized human ectocervical cells (ECT1, ATCC, CRL-2614) were cultured in keratinocyte serum free medium (17005-042, Gibco, Grand Island, NY) with bovine pituitary extract and hEGF supplementation as recommended by ATCC under 5% CO2 at 37°C.

    Techniques: Blocking Assay, Infection, Western Blot